Table 3.
Primer sequences for quantitative real-time PCR
| Gene name | Primer sequences-forward (F) and reverse (R) | Target Tissue |
|---|---|---|
| 1-aminocyclopropane-1-carboxylate oxidase | F-TGCAGGTGCCGGGTAGAT R-ATGTCCCTAGCCTCGTTCTACAAC |
Fruit |
| Abscisic stress ripening | F-CCAATTTCACAACCTCGCTCTA R-TGGTGATCGGAAAAGGAAAAA |
Bud |
| Amino acid selective channel protein | F-AATGGAGTATGGGATGGAGAGAGT R-CCCCGAGCATTGCATTCTT |
Bud |
| Anthocyanidin synthase | F-TTCGTGGGCGGCTTTCT R-GAGCGGCTTCAGGATGATCT |
Fruit |
| Arginine decarboxylase | F-CGCTTCGTGCGAGCTAGATA R-TGCAATAGTCTCTCACCACTCGAT |
Bud |
| Aspartic proteinase | F-GAGTGCCATCGGTATAGCACAA R-CACAGGCAGAGCCCCAAA |
Fruit |
| Chalcone synthase | F-AGGCCTTCCAGCCTTTGG R-GGTGGGCGATCCAGAAGAT |
Fruit |
| Cysteine protease-like protein | F-CAACGGTTGAGACCATGGAAT R-TGCAGAGGTCCCCATGCT |
Fruit |
| Dehydrin-14 kDa | F-CGCGGCGATTAGATCGAA R-AGCTAGCGTAGAGGCGGAAA |
Bud |
| Flavonoid 3-hydroxylase | F-GCCGGTGCAACCCACTT R-GGCCGTAGTTGGCGAAAAC |
Fruit |
| Galactinol synthase | F-AAGGGTGTGGTGGGATTGG R-CCAAAGGGTACGCGC CTT |
Bud |
| Glutathione peroxidase | F-TCTAGCATCCTTGACGGTGAAG R-GGCAAGCCAATCCGAGAAG |
Fruit |
| High mobility group family protein | F-CGTCATGAAAGGAGGTAAATCCA R-TTTCGCAGTTTTCTTCACCGATA |
Bud/Fruit housekeeping gene |
| Hypothetical protein | F-CCTGCTGCATCAAGCTGTTG R-TGGCTGGAAGCACTCACTGT |
Bud/Fruit housekeeping gene |
| Lipid transfer protein | F-CCAAGGTGAAATGAGCAAGGA R-GCTTCATCCCGCGACATG |
Bud |
| Lipoxygenase | F-CCAGCTGGATGTGGCAACTT R-ACGCCTGCGTCATTGGA |
Bud |
| Metallothionein | F-ACCCTGACATGAGCTTCTCG R-ACCCAAATCTCTGCTTGCTG |
Bud housekeeping gene |
| Pectate lyase | F-GGTTGCCGGTCCCACAA R-GAGCATCAATGCGTCAAGGA |
Fruit |
| Pointed first leaf | F-CGTTTTGCCAACATCGTTTG R-GCTCACCAGCCCTCTTGTTC |
Bud |
| Pyrophosphate-dependent phosphofructokinase | F-GCCCTTAACAACCGCTACATCA R-TCTGCTACTGGGCCAACAAAT |
Bud |
| S-adenosyl-1-homocysteine hydrolase | F-CACCGGATCGCTTCACATG R-CGGTGAGGGTTTCGATAAGG |
Fruit housekeeping gene |