Skip to main content
. 2012 Jun 19;7(6):e39359. doi: 10.1371/journal.pone.0039359

Table 3. TaqMan probes used for the quantification of gene expression by qRT-PCR.

Gene family Gene TaqMan catalog number
ER stress HYOU1 Hs00197328
PDIA4 Hs01115905
PPIB Hs00168719
HSPA5 Hs00946084
ATF4 Hs00909569
ATF6 Hs00232586
VCP Hs00200205
Integrins ICAM1 Hs00164932
THBS1 Hs00962908
SVEP1 Hs00295944
CTGF Hs00170014
Cell cycle TP53 Hs01034249
CDK2AP2 Hs00366670
CDKN1A Hs00355782
Oligotransferases SRPR Hs01112418
SEC61A1 Hs01037684
Mitochondria MT-ND6 Hs02596879
MT-CO1 Hs02596864
MT-CYB Hs02596867
Other VEGFA Hs00900055
MANF Hs00180640
IL6 Hs00985639
House keeping GAPDH Hs99999905

Thermocycler parameters were 10 min denaturation at 95°C, followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. Values were interpolated into a standard curve to calculate absolute expression. These values were then divided by the expression of GAPDH in the same samples to correct for potential pipetting errors.