Table 3. TaqMan probes used for the quantification of gene expression by qRT-PCR.
Gene family | Gene | TaqMan catalog number |
ER stress | HYOU1 | Hs00197328 |
PDIA4 | Hs01115905 | |
PPIB | Hs00168719 | |
HSPA5 | Hs00946084 | |
ATF4 | Hs00909569 | |
ATF6 | Hs00232586 | |
VCP | Hs00200205 | |
Integrins | ICAM1 | Hs00164932 |
THBS1 | Hs00962908 | |
SVEP1 | Hs00295944 | |
CTGF | Hs00170014 | |
Cell cycle | TP53 | Hs01034249 |
CDK2AP2 | Hs00366670 | |
CDKN1A | Hs00355782 | |
Oligotransferases | SRPR | Hs01112418 |
SEC61A1 | Hs01037684 | |
Mitochondria | MT-ND6 | Hs02596879 |
MT-CO1 | Hs02596864 | |
MT-CYB | Hs02596867 | |
Other | VEGFA | Hs00900055 |
MANF | Hs00180640 | |
IL6 | Hs00985639 | |
House keeping | GAPDH | Hs99999905 |
Thermocycler parameters were 10 min denaturation at 95°C, followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. Values were interpolated into a standard curve to calculate absolute expression. These values were then divided by the expression of GAPDH in the same samples to correct for potential pipetting errors.