Figure 7. E2 inhibited TNF-α-induced MCP-1 and CINC-2β mRNA expression in RASMCs through ERβ.
Cells were grown to subconfluence (≈95%) in 6-well plates, deprived of serum for 24 hrs, pretreated with E2 (10−7 M), DPN (10−7 M) or vehicle for 24 h, and then treated with TNF-α (1 ng/ml) for an additional 1 hr. MPP (10−6 M), or R, R-THC (10–6 M) was given to cells at 1h before E2 treatment in some experiments. Data, expressed as means±SEM, are from real-time quantitative RT-PCR assays and are normalized by 18 S RNA. Data for MCP-1 and CINC-2β are standardized to the mean mRNA level of the TNF-α-treated RASMCs. *p<0.05 vs. respective vehicle-treated RASMCs; #p<0.05 vs. respective TNF-α-treated RASMCs.