Table 3. Vertical transmission of Rabensburg virus in three strains of Culex pipiens. ^* .

Mosquito Strain	Oviposition dpi	No. of pools tested†	MLE [positive pools/total]
CpUS
OP1 Larvae	7	151	46.5 [32/151]
OP2 Larvae	14	69	15.0 [5/69]
OP3 Larvae	21	22	0.00 [0/22]
		MLE Combined	32.6 [37/242]
CpEU
OP1 Larvae	7	43	40.3 [8/43]
OP2 Larvae	14	23	47.8 [5/23]
OP3 Larvae	21	2	0.00 [0/2]
		MLE Combined	41.5 [13/68]
CxM
OP1 Larvae	7	35	5.78 [1/35]
OP2 Larvae	14	9	49.0 [2/9]
OP3 Larvae	21	5	97.1 [2/5]
		MLE Combined	21.3 [5/49]

^*MLE, maximum likelihood estimation of infection rates; CpUS, a United States strain of Culex pipiens that contain genetic signatures of both form pipiens and form molestus; CpEU, a European strain of Culex pipiens form molestus; CxM, a United States strain of Culex pipiens form molestus; OP, oviposition; dpi, days post infection; Vertical transmission of RABV was observed in two independent experiments for CpUS and CxM and in one out of two independent replicates for CpEU. All surviving parental females were pooled in groups of five, screened for viral infection, and all pools were RABV positive.

^†pool size = 5.