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. 2012 Jun 19;7(6):e38733. doi: 10.1371/journal.pone.0038733

Figure 4. Co-localization of cytokine receptors with lipid rafts of resting CD4 T-cells before and after squalene treatment.

Figure 4

Resting CD4 T-cells from individual F1 mice (n = 5/group) before and 7 days after squalene treatment (180 µg/mouse) were analyzed for interleukin receptor expression and distribution at the single-cell level by CLSM. Cells were stained with IL-4Rα-, IL-12Rβ2-, or IL-2Rα-PE conjugates, and co-stained for GM1 ganglioside by CTB- FITC conjugate and for nuclei with DAPI. First column indicates single-channel color for DAPI staining (blue), second column indicates GM1 staining (green), third column indicates interleukin receptor (IL-Rs) staining (red), and last column indicates merged channels at X63 magnification. Top-two rows, indicate cells from untreated (upper row) and squalene treated mice (lower row) stained for IL-4Rα. Middle-two rows, indicate cells from untreated (upper row) and squalene treated mice (lower row) stained for IL-12Rβ2. Bottom-two rows, indicate cells from untreated (upper row) and squalene treated mice (lower row) stained for IL-2Rα. Arrows indicate presence of IL-Receptor co-expression with the GM1 resident of lipid rafts. Enlargements of the merged channels are depicted to the right along with two different angles of the membrane for each IL-Receptor at X220 magnification. Shown are representative images in one of three experiments.