Table 1. Performance of silica adsorption and oligonucleotide capture methods with different size pools of S. ochraceum.
Pool size | Sample | Silica Positive | Mean Silica ELISA OD (range)* | Capture positive | Mean Capture ELISA OD (range)* |
50 | Heads | 100% (3/3) | 0.286 (0.142–0.476) | 100% (3/3) | 0.647 (0.637–0.667) |
100 | Heads | 0% (0/3) | 0.088 (0.071–0.098) | 100% (3/3) | 0.662 (0.659–0.667) |
150 | Heads | 0% (0/3) | 0.082 (0.076–0.094) | 100% (3/3) | 0.638 (0.630–0.645) |
200 | Heads | 0% (0/3) | 0.080 (0.078–0.081) | 100% (3/3) | 0.603 (0.600–0.608) |
50 | Bodies | 100% (3/3) | 0.119 (0.104–0.134) | 100% (3/3) | 0.113 (0.110–0.119) |
100 | Bodies | 100% (3/3) | 0.116 (0.104–0.127) | 100% (3/3) | 0.192 (0.190–0.193) |
150 | Bodies | 100% (3/3) | 0.120 (0.104–0.149) | 100% (3/3) | 0.261 (0.260–0.262) |
200 | Bodies | 0% (0/3) | 0.071 (0.062–0.081) | 100% (3/3) | 0.231 (0.230–0.232) |
*: mean of positive control wells = 0.431 (range 0.369–0.492).