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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Leukemia. 2012 Feb 10;26(7):1537–1546. doi: 10.1038/leu.2012.38

Figure 3. Expression of WT and mutants C/EBPα modifies Proliferation and Apoptosis of Human Hematopoietic Progenitors.

Figure 3

(A) Analysis of Cell Cycle of transduced cells at 2 weeks in culture. Different Cell Cycle Stages were identified by FACS according expression of Ki67 and DNA amount by DAPI. Cell Cycle profile of Human Lin- transduced by Control (white bars), WT C/EBPα (dark grey), N-terminal (light grey) and C-terminal (black) is shown. Data are from three independent experiments done for triplicate. (B) Transduction by different C/EBPα forms increases Human Lin- cell survival. Representative Dot-Plot representations of DAPI and Annexin V distinguish alive (left square) and dead cells (right square). Means ± SD are indicated. (C) Quantitative real-time PCR analysis of Bcl-2 in transduced Human Cells. Sorted transduced cells were analyzed in two independent experiments. Results are expressed as mean ± SD, with significant differences of p ≤ 0.05, p ≤ 0.01 or p ≤ 0.001 are indicating by (*), (**) or (***) respectively.