Figure 5. Modification of Myeloid Differentiation by of WT and mutants C/EBPα.

(A). Human Lin⁻ cells were cultured in Myeloid Conditions for two weeks. FACS analysis evaluated percent of different populations of Myeloid Lineage in transduced cells: Monocytes (hCD33⁺/hCD14⁺, grey), Granulocytes (hCD33^low/hCD15⁺, white) and Granulocytic precursors (hCD33⁺/hCD15⁺, black). (B) Percentage of CD34+CD38− (white) and CD34+CD38+ (black) in transduced cells after two weeks of culture in myeloid conditions. Percent of each subset is indicated. Data show triplicates of three independent experiments. Significant differences of p ≤ 0.05 or p ≤ 0.001 are indicating by (*) or (**) respectively.