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. 2012 Jun 19;7(6):e39170. doi: 10.1371/journal.pone.0039170

Figure 2. Peripheral activation of NK cells results in the selection of lymph node DCs with higher T cell activating capabilites.

Figure 2

Draining lymph node DCs of mice injected subcutaneously with MHCnegcells were sorted and cultured in the presence of allogeneic splenocytes previously labeled with CFSE. A: Splenocytes were cultured alone (No stimulus) or with 10% of highly purified lymph node DCs from mice NK cell depleted (10% DC draining LN + anti-asialo GM1) or control (10% DC draining LN) before administration of MHC-negative target cells. CFSE dilution of the splenocytes at 6 days of culture is shown. NK cell depletion compromises the induction of proliferation by LN DCs. Data are representative of four independent experiments summarized in panel B: ▪ = 10% DC draining LN; ▴ = 10% DC draining LN + anti-asialo GM1. * =  p<0,02.