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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: J Immunol. 2012 Apr 13;188(10):5041–5053. doi: 10.4049/jimmunol.1101106

Figure 4. Multifunctionality of P16 and P79 specific CD4+ T cells.

Figure 4

Mice were immunized with the same regimen as described in Figure 1. Two weeks post-prime with AdHu5-PcAMA1 (day 14) and two weeks post-boost with MVA-PcAMA1 (day 70), splenocytes were re-stimulated with the P16 and P79 peptide or no peptide (Unstim) for 5h. Cells were surface stained for CD4 and intracellularly stained for IFN-γ, TNFα and IL-2, and assessed by flow cytometry to determine the % of responding cells per total CD4+ T cell subset. Toal responses of each cytokine to P16 (A) and P79 (B) are shown at each time-point. The multifunctionality or “quality” of CD4+ T cells was analyzed using the SPICE software.