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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Immunol. 2012 Apr 2;188(9):4663–4670. doi: 10.4049/jimmunol.1103472

Figure 6. C-mut are escape mutations and markers for immunogenic epitopes.

Figure 6

(A) Magnitude of IFN-γ responses to 18-mer HIV-1 consensus peptides. PBMCs from patients expressing HLA-C alleles associated with each C-mut were used in an ELISpot assay. Each bar represents one patient. HLA-C type of each subject is shown above the bars; the HLA-C alleles associated with C-mut are in bold (Fig. 2). Two to five responding patients were identified for each peptide. (B) TNF-α and IFN-γ production is CD8+ T cell mediated, HLA-Cw*01:02-restricted and peptide-specific. A short-term CTL line was re-stimulation with VL8-pulsed .221HLACw*01:02 transfectants (left). Untransfected VL8-pulsed .221 cells were used as control (right). Numbers indicate percentage of positive cells. Gated on CD3+ T cells. (C and D) S173T and T470A substitutions are escape mutations. Peptide titration assay with CTL line or PBMCs in presence of decreasing concentrations of adapted (white) and nonadapted (black) (C) VL-8 or (D) TAPPEESFRFGEETTTPSQK peptides in an ELIspot assay. Data representative of two independent experiments. Statistical analyses were performed using a two-tailed t-test. Significant differences are indicated by the presence of *.