Figure 4.

I.n. immunisation induces higher gp140-specific IgA responses than i.d immunisation. Mice were immunised i.n. (open symbols) or i.d. (black symbols) three times with 10 μg gp140 alone or with 5 μg TSLP or CT. (A, C) Anti-gp140 IgG and (B, D) anti-gp140 IgA were measured by ELISA (A, B) in serum 3 weeks after priming (first immunisation), first boost (second immunisation) and final immunisation (third immunisation) and (C, D) in vaginal lavage, faecal extracts and nasal lavage 3 weeks after the final immunisation. Data are shown as endpoint titres, with lines representing geometric means of n=5 mice; data are representative of two experiments. ^***p<0.001, one-way analysis of variance (ANOVA) with Tukey's multiple comparison post test.