Figure 5.

TSLP induces sustained humoral and cellular immune responses to gp140. Mice were immunised four times i.n. with 10 μg gp140 alone (▪) or with 5 μg TSLP (○) or CT (◊). The fourth immunisation was given 6 months after the third immunisation. Anti-gp140 IgA and IgG were measured by ELISA in (A, B) serum and (C, D) vaginal lavage before every immunisation and 3 weeks after the final immunisation. (E) CN54 gp140 IgA and IgG ASC were measured by B-cell ELISPOT in spleens from mice 3 weeks after the final immunisation. Antigen-specific T-cell proliferation of splenocytes was assessed after in vitro stimulation with gp140 after 120 h using (F) ³H-thymidine and (G) CFSE dilution. Data are shown as mean of n=5 mice+SD, experiment was performed once. ^*p<0.05, ^**p<0.01, ^***p<0.001, one-way analysis of variance (ANOVA) with Tukey's multiple comparison post test.