Figure 2.

Earlier and enhanced accumulation of CD4⁺ T cell and cytokine-producing CD4⁺IFNγ⁺ cells in the lungs of infected IL-10^−/− as compared with WT mice. (A) The total number of CD4⁺CD3⁺ T cells present in the indicated organs of WT (open bars) and IL-10^−/− (closed bars) BALB/c mice infected with Mtb was determined by flow cytometry and calculated based on the total cell counts acquired at each time point for each individual mouse. The percentage of CD4⁺IFNγ⁺ T cells (Supporting Information Fig. 1B) and total numbers of CD4⁺IFNγ⁺ cells (B) were determined following restimulation of whole organ homogenates ex vivo. Cells were stained with the appropriate Ab and CD4⁺IFNγ⁺ levels determined by flow cytometry according to the gating strategy shown in Supporting Information Fig. 1A. Data are mean±SEM and are representative of at least two independent experiments (n=3–4 mice per group). ^*p<0.05 (unpaired Student's t-test); ND, not done.