Figure 3.

Following Mtb infection, IL-10^−/− mice have an earlier and enhanced production of proinflammatory cytokines in the lungs as compared with WT mice. Lung tissue from Mtb-infected WT (open bars) and IL-10^−/− BALB/c (closed bars) mice was isolated, homogenized and restimulated with PPD after which supernatants were removed and screened by Multiplex assay for the indicated cytokines (see also Supporting Information Table 1). Data are mean±SEM and are representative of at least two independent experiments (n=3–4 mice per group). ^*p<0.05; ^**p<0.01; ^***p<0.001 (unpaired Student's t-test); ND, not done; <, below detection limit of the assay.