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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: Virology. 2012 Jun 2;431(1-2):12–20. doi: 10.1016/j.virol.2012.05.009

FIGURE 3. Contributions of motif F arginines to RNA synthesis.

FIGURE 3

(A) Sequence-based alignment of motif F from VP1 of representative strains of RVA-RVD and RVF-RVH, and structure-based alignment of RVA VP1 with reovirus λ3 (Reo). Residues that are identical for all species of RV are denoted with an asterisk. Residues identical for all RdRps are denoted with grey text. (B) Representative autoradiograph of radiolabeled dsRNA products from an in vitro dsRNA synthesis assay (upper) and Coomassie-stained polyacrylamide gel showing relative amounts of input VP1 protein (lower). Mutations engineered in VP1 are indicated. (C) Graph of relative amounts of products of dsRNA synthesis assays. Error bars represent SEM for results of six experiments. Means are indicated above bars. *, P < 0.01.