Fig. 2.

Hippocampal Aβ levels and tau immunoreactivity. A) Top, representative examples of CA1 area photomicrographs taken from animals fed either control diet (CTRL) or PIO-enriched diet (PIO) and stained using WO2 antibody against the Aβ peptide. Bottom, quantification of WO2 staining shows decreases in integrated Aβ density, and in the area covered by Aβ positive cells (percent). B) Results from Sandwich ELISA method to measure soluble (left) and insoluble (right) Aβ fractions. While not significant, a trend was seen in the soluble fraction decreasing with PIO treatment (p = 0.07). C) Photomicrographs of hippocampal sections stained with PHF-1 antibody to monitor the presence of hyperphosphorylated tau. A significant reduction in tau immnunoreactivity was seen (see text). Data represent Mean ± SEM, T-test, *p < 0.05.