Fig. 4.

Membrane hyperpolarization measures. A) Example of afterhyperpolarization (top) elicited intracellularly with three action potentials and recorded in a 3×Tg-AD mouse fed the control diet (action potentials are truncated). Bar graphs show the amplitude of the medium afterhyperpolarization (mAHP) and slow AHP (sAHP) as well as the duration of the duration recorded in mice fed either control diet (CTRL) or PIO-enriched diet (PIO) (T-test, p > 0.05). Note that the AHPs are particularly small in this animal model. B) Top, train of action potentials elicited synaptically with repetitive stimulation for 10 s at 7 Hz. Bottom, PIO animals showed a significant increase in maximal membrane hyperpolarization (measured from baseline – dashed line, Δ mV) at the three frequencies tested. *p < 0.05, Two-way ANOVA. Data represent Mean ± SEM.