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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Hepatology. 2012 Jul 12;56(3):982–993. doi: 10.1002/hep.25701

Fig. 3. SAMe and MTA treatment lowered Ubc9 protein levels in HepG2, Huh-7, RKO and MCF-7 cells.

Fig. 3

Cells were treated with SAMe (2mM) or MTA (1mM) for 24 hours. (A) Quantitative real-time PCR measured mRNA levels of Ubc9. Results are expressed as fold of control (mean ± SEM) from 3 independent experiments. (B) Western blot analysis using whole cell lysates (10μg) measured Ubc9 protein levels. The values are expressed as % of control (mean ± SEM) from 3 independent experiments. *P<0.001 vs. control of HepG2, *p<0.001 vs. control of Huh-7, *p<0.01 vs. control of RKO and *p< 0.01 vs. control of MCF-7 cells.