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12% Bis-Tris SDS-PAGE analysis of TMD-5 after NMR titration. The ratio of compound 1 to TMD-5 in TMD-5 + 1 sample shown here is 4:1. Compound 1 disrupts the TMD-5 trimer (MW 10.2 kDa ) and causes TMD-5 monomer (3.4 kDa) formation. It should be noted that the loading amount of TMD-5 in lane 2 was greater than that of lane 3.
