A, Western blot analysis of the levels of erbB2/EGFR and their downstream molecules in gallbladder from non-treated BK5.erbB2 mice (left); treated gallbladders diagnosed as PR (middle); treated gallbladders diagnosed as PG (right). B, ErbB2 and p-erbB2 expression in gallbladder from non-treated BK5.erbB2 mice (upper); treated gallbladders diagnosed as PR (middle); treated gallbladders diagnosed as PG (lower). (scale bar, 40µm); C, Effect of PCI-24781 on the level of erbB2 in primary cultured BK5.erbB2 gallbladder cells treated with PCI-24781.(scale bar, 20µm); D, Relative erbB2 mRNA levels in gallbladders treated with PCI-24781. Gallbladders from non-treated BK5.erbB2 mice (left); treated gallbladders diagnosed as PR (middle); treated gallbladders diagnosed as PG (right).