Skip to main content
. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Struct Biol. 2012 May 5;179(1):68–75. doi: 10.1016/j.jsb.2012.04.020

Figure 2. Equipment model, setup and inkjet head positioning.

Figure 2

A) 3D model of equipment components (notations provided below), comprised of the plunge axis, rear and side-view optics, dispense head and positioning stages. B) Close-up model view of the inkjet dispense head positioned in proximity to the EM grid and the specimen dispense process monitored by the rear and side-view optics. C) Integrated equipment setup for inkjet dispense and vitrification (notations provided below). D) Front view of the inkjet dispense head utilized for picoliter to nanoliter specimen dispensing. E) Side view showing the positioned EM grid and inkjet dispense head prior to specimen dispense. F) Time-lapse sequence of a single droplet of specimen (~32 pL) dispensed from the inkjet head. Notations in (A–C): (g)–EM grid, (h)–inkjet dispense head mounted on pneumatic stage, (j)-manual pneumatic plunging valve, (m)-manual 3-axis positioner holding plunge assembly, (o)-rear-view objective lens, (p)-pneumatic plunge assembly, (q)-liquid ethane reservoir, (r)-rear-view camera, (s)-side-view camera, (t)-tweezers holding EM grid, (u)-LED strobe light, (v)-manual rotation and z-axis stage and (w)-manual 2-axis stage for positioning dispense head.