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. 2012 May 22;61(7):967–978. doi: 10.1007/s00262-012-1282-9

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© The Author(s) 2012

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Guidelines partially harmonized ICS performance in phase 2. a The gating instructions provided to the participants for the second-phase ICS proficiency panel. First, the lymphocytes are gated (R1), then the CD3 population (R2), followed by plotting CD4 versus CD8 to gate on the CD8 (including the CD8dim) cells (R3), which is called the CD3+ CD4− cell population, and finally the IFNγ-producing CD3+ CD4− T cells can be gated. b The average frequency (+SD) of IFNγ-producing CD3+ CD4− T cells is depicted for each donor (D) in the negative control samples (black bars) and after stimulation with the CMV (top) or FLU (bottom) peptide (white bars). c The CV values per donor (D)–antigen (CMV or FLU) combination are depicted for those 6 laboratories participating in both phases 1 (white bars) and 2 (black bars)