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p-GSK3β(S9) immunostaining in CAH of intact Pten+/− mice (A) and Pten+/− mice that had undergone oophorectomy (B). Original magnification, ×20. Phosphorylation at Ser 9 was significantly up-regulated in the mice that had undergone oophorectomy (C). Statistical analysis was performed with the Mann–Whitney test. **P = 0.005.
