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Immunostaining with Ki-67 (A–D) and cyclin D1 (E–H) on uteri from Pten+/+;ERα+/+ (A and E), Pten+/+;ERα−/− (B and F), Pten+/−;ERα+/− (C and G), and Pten+/−;ERα−/− (D and H) mice. Invasive carcinomas in the Pten+/−;ERα−/− mice exhibit reduced number of Ki-67-positive cells but increased staining of cyclin D1. Original magnification, ×20.
