Figure 6.

Identification of 5′- and 3′-ends of hisR and cysT transcripts in the wild-type strain (SK10153) and the Δrnt rph-1 double mutant (SK10148) by sequencing of cDNA clones obtained from 5′- to 3′-self-ligated transcripts. Schematic presentations of hisR and cysT tRNAs (rectangles) with the 5′ upstream and 3′ trailer sequences are shown directly above respective tRNA clones. The endonucleolytic cleavages (open scissors) upstream of 5′ and downstream of 3′ mature end (CCA terminus) are as previously identified (1,2,4). The 5′- and 3′-ends (without non-templated a's) are shown as inverted arrows above the sequences. The 3′-ends with non-templated a's are shown as bent upward arrows below the sequences. The source of some a residues in hisR (italicized a, see text) could not be unequivocally determined. The numbers directly above each arrow represent the number of independent clones identified with a respective 5′- or 3′-end. The numbers in parenthesis represent the number of independent clones identified with non-templated a residues. Some of the tRNA transcripts are truncated at 3′-ends by 5–16 nt from CCA terminus (indicated by a star).