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. 2012 Jan 28;40(10):4589–4603. doi: 10.1093/nar/gks006

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Characterization of poly(A) tails associated with tRNAs (A) and mRNAs (B) using northern analysis. Total RNA isolated from SK9124 (rph-1/pBMK11) after 0, 15 and 45 min of IPTG (350 µM) induction was either treated (+) or not treated (−) with RNase H in presence of oligo(dT)₂₀ and separated on 6% PAGE as described in the Materials and Methods. The blots were probed with either oligod(dT)₂₀ (to detect poly(A) tails) or probed for hisR, or lpp. A star indicates polyadenylated transcripts. The length of mature species is noted below each transcript.