Table 1.
Strain | Genotype | Relative amounts of poly(A) tailsa |
|
---|---|---|---|
<10 nt | >10 nt | ||
SK10153 | thyA715 | 1 | 1 |
MG1693 | rph-1 thyA715 | 1.3 ± 0.2 | 1.4 ± 0.3 |
SK10592 | Δrnt thyA715 | 4 ± 1 | 0.4 ± 0.1 |
SK10148 | Δrnt rph-1 thyA715 | 30 ± 5 | 0.5 ± 0.1 |
SK10020 | ΔpcnB Δrnt rph-1 thyA715 | 0.6 ± 0.2 | ND |
aThe level of poly(A) tails was determined by separately integrating all bands >10 nt and ≤10 nt as shown in Figure 1 using ImageQuant TL 5.2 software (see ‘Materials and Methods' section). The pixel counts for the respective group of bands in the wild-type control were set at one.
ND = not detected.