Table 2.
Effect of absence of RNase T and RNase PH on selected tRNAs and rRNA
| tRNA isotype/rRNA | No. of Loci | Gene(s) | Percentage of immature transcriptsa |
Presence of poly(A) tailsb | |
|---|---|---|---|---|---|
| Δrnt rph-1 | Δrnt ΔpcnB rph-1 | ||||
| Arg2 | 4 | argQ, argV, argY, argZ | 45 ± 3 | 14 ± 2 | Yes |
| Asn | 4 | asnT, asnU, asnV, asnW | 27 ± 4 | 8 ± 2 | Yes |
| Cys | 1 | cysT | 44 ± 0 | 31 ± 2 | Yes |
| Glu2 | 4 | gltT, gltU, gltV, gltW | 26 ± 3 | 17 ± 0 | Yes |
| His | 1 | hisR | 71 ± 2 | 59 ± 1 | Yes |
| Leu1 | 4 | leuT, leuQ, leuP, leuV | 41 ± 1 | 20 ± 3 | Yes |
| Leu2 | 1 | leuU | 20 ± 2 | 12 ± 2 | Yes |
| Leu3 | 1 | leuW | 54 | 44 | Yes |
| Leu4 | 1 | leuZ | 38 | 29 | Yes |
| Leu5 | 1 | leuX | 61 ± 1 | 30 ± 5 | Yes |
| Lys | 6 | lysQ, lysT, lysV, lysW, lysY, lysZ | 24 | 9 | Yes |
| Met f1, Met f2 | 4 | metZ, metW, metV, metY | 0 | 0 | No |
| Met m | 2 | metT, metU | 58 ± 0 | 41 ± 3 | Yes |
| Phe | 2 | pheU, pheV | 67 ± 3 | 45 ± 5 | Yes |
| Pro1, Pro2, Pro3 | 3 | proK, proL, prom | 0 | 0 | No |
| Ser1 | 1 | serT | 50 | 23 | Yes |
| Ser3 | 1 | serV | 56 | 42 | Yes |
| Ser5 | 2 | serW, serX | 36 | 9 | Yes |
| Val1 | 5 | valU, valX, valY, valT, valZ | 53 | 48 | Yes |
| Val2A, Val2B | 2 | valV, valW | 53 | 38 | Yes |
| 5S | 8 | rrfA, B,C,D,E,F,G,H | 100 ± 0 | 100 ± 0 | Yes |
aRepresents the fraction of immature species out of total (mature, immature, and polyadenylated species, see Figure 3) RNA species detected. Data, with standard deviations, represent the average of at least three independent experiments.
bPoly(A) tails were scored as being present if the immature species observed in the Δrnt rph-1 strain were shortened in the ΔpcnB Δrnt rph-1 triple mutant.