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. 2012 Jan 23;40(10):4626–4640. doi: 10.1093/nar/gks026

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Mammalian mirtrons and simtrons function in silencing. Luciferase expression in HEK-293T cells transiently co-transfected with pmiRGLO with matching miRNA target sequences or mis-match miRNA target sequences and two different concentrations (0.4 or 1 µg) of the wt or splicing-deficient (Δss) minigene. Horizontal dotted line represents the normalized mismatch control value. The following equation was used to analyse the data: [(Luciferase Target/Renilla)_{miR_match}]_avg/[(Luciferase Target/Renilla)_{miR_mismatch}]_avg. Each sample was analysed in triplicate. Bars represent the average ± SEM, n = 5 independent experiments.