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. 2012 Feb 6;40(10):4701–4710. doi: 10.1093/nar/gks032

Figure 3.

Figure 3.

The cassette functions predictably in different gene contexts. (A) RT–PCR detection of spliced products for fLUC and abscissic acid 8’-hydroxylase (CYP707A3) harboring the P5SM cassette inserted within the E/R, K/R or E/S sequence contexts, upon induction with AtL5 (+) or LUC as a control (−). (B) Relative amounts of SP-I transcript for fLUC constructs determined by RT–qPCR. Data are averages with number of biological replicates (n) and standard deviations shown. The p-value was determined using the paired t-test. (C) Luciferase activity in relative light units of fLUC constructs normalized to total protein upon induction with AtL5 (+) or control (−). Fold induction by AtL5 is shown at the top of each bar. (D) RT–PCR detection of spliced products for PSY harboring the P5SM cassette inserted within the C/R sequence context upon induction with AtL5 (+) or LUC as a control (−). A schematic representation of the identified aberrant spliced products (right) shows cryptic splicing from a proximal GU in the coding sequence (sequences are shown in Supplementary Figure S6).