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. 2012 Feb 6;40(10):4701–4710. doi: 10.1093/nar/gks032

Figure 4.

Figure 4.

Rational design of the P5SM RNA element leads to generation of an orthogonal splicing cassette. (A) Fold induction of protein expression quantified by eGFP fluorescence for reporters harboring the OsP5SM or HyP5SM RNA elements within the splicing cassette. Induction with AtL5 or OsL5 was each measured in comparison to induction with LUC as a control. (B) Fluorescence of eGFP-HyP5SM without coinfiltration with DsRed2 and with subtraction of background autofluorescence for individual leaf samples. Fold induction by OsL5 relative to induction with LUC as a control is shown. Background subtraction gives a negative fluorescence value (asterisk) for leaf 4 in the absence of OsL5 induction, so the fold induction could not be determined for this sample. (C) Representative whole leaf scans of eGFP fluorescence for reporters harboring OsP5SM or HyP5SM. The right leaf halves coexpressed AtL5 or OsL5 and the left halves coexpressed LUC as a control. All samples except for the one labeled ‘No DsRed2’ also coexpressed DsRed2 on both leaf halves. (D) RT–PCR detection of spliced products for eGFP-OsP5SM and eGFP-HyP5SM upon induction with AtL5, OsL5, or LUC as a control.