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. 2012 Jan 2;40(10):4422–4431. doi: 10.1093/nar/gks034

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — FDM1 and FDM2 play redundant and essential roles in RdDM. (A) Reduced DNA methylation at AtSN1, IGN5 and FWA SINE in fdm1-1 fdm2-1. HaeIII-digested genomic DNAs from various genotypes were used for PCR amplification of AtSN1 and IGN5, whereas AvaII-treated genomic DNAs were used for the amplification of FWA SINE. Amplifications of undigested genomic DNA are used as loading controls. Col: WT plants. (B) Reduced DNA methylation at AtSN1 and siR02 loci in fdm1-1 fdm2-1. McrBC-digested and -undigested DNAs (control) were used for the amplification of AtSN1 and siR02. (C) Reduced DNA methylation at MEA-ISR in fdm1-1 fdm2-1. MspI-digested genomic DNAs from various genotypes were probed for MEA-ISR. Bands representing methylated (ME) or unmethylated (UM) DNA are indicated. (D) Reduced DNA methylation at 5S rDNA locus in fdm1-1 fdm2-1. HaeIII, HpaII or MspI-digested genomic DNAs from various genotypes were probed for 5S rDNA. (E) Reduced DNA methylation at AtMU1 locus in fdm1-1 fdm2-1. HaeIII-digested genomic DNAs were probed for AtMU1. The three undigested bands presented in Col (WT) but not in nrpe1-1 and fdm1-1 fdm2-1 were indicated by arrows. (F) Unaffected DNA methylation at 180 bp centromeric repeats in fdm1-1 fdm2-1. Following HpaII, MspI or HaeIII treatment, genomic DNAs from various genotypes were probed for 180-bp centromeric repeats.