Figure 5.

FDM1 binds dsRNAs with 5′ overhangs. (A) The two purified proteins used in the binding assay, GST and GST-FDM1ΔZH (truncated FDM1 containing XS and SMC domain) were resolved in SDS–PAGE gel and stained with coomassie Blue. The protein molecular weights are indicated on the right. (B and C) RNA-binding assays of FDM1 with various probes. The structure of various probes is shown on the right. Asterisk indicates radioactive labeled RNA strand. Approximately 50 µg of protein was used for the binding assay. For dsRNAs with 5′ overhang, 1×, 10× and 150× unlabeled RNAs of the same sequence were used for the competition assay.