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. 2012 Jan 28;40(10):4358–4367. doi: 10.1093/nar/gks037

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Influence of Fis on promoter activity in vivo. (A) β-Galactosidase expression was measured in E. coli CSH50 wild-type and fis⁻ strains containing P_tin7-lacZ fusion reporter construct. β-Galactosidase activity in wild-type cells was 4- to 5-fold lower than in the fis⁻ strain. (B) β-Galactosidase activity measured in CSH50 wild-type and fis⁻ strains containing the C encoding plasmid in addition to the plasmid borne P_tin7-lacZ fusion reporter construct.