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. 2012 Jan 28;40(10):4358–4367. doi: 10.1093/nar/gks037

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Effect of Fis on closed complex formation on various promoter fragments. (A) P_tin7 (−58 to +20 relative to +1 start point) (lane 1) was incubated with increasing concentrations of Fis as indicated (lanes 2–6). Subsequently, samples were incubated with 20 nM RNAP to allow closed complex formation (lanes 7–12). Mutant promoter fragments namely P_tin7FBS-49-38 (Fis binding sites at −49 and −38 mutated) (B), P_tin7FBS+3 (Fis binding site at +3 mutated) (C), P_tin7FBS-49-38+3 (all three Fis binding sites at −49, −38 and +3 mutated) (D) were also examined for the effect of Fis on closed complex formation at the respective mutant promoters. On the left are schematic depictions of occurrence or disruption of Fis binding sites in the respective fragments. F denotes free promoter DNA. DNA–protein complexes are indicated.