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Effect of disrupting Fis binding sites on Fis-mediated repression. pLW4tin7 and variants containing mutations in Fis binding sites were introduced into isogenic strains CSH50 fis− and CSH50 wild-type (fis+)and β-galactosidase activity was measured. Fold repression (the ratio of the β-galactosidase activity in fis− strain to that in fis+ strain) was plotted.
