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. 2012 Feb 8;40(10):4483–4495. doi: 10.1093/nar/gks041

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Transcriptional regulation of DDR genes by CUX1. (A) MCF7 cells were transfected with CUX1-specific siRNA. Top panel: mRNA and protein levels of CUX1 are shown following knockdown. Bottom panel: mRNA levels of DDR gene targets are shown. All mRNA levels are normalized to Hypoxanthine-guanine phosphoribosyltransferase (HPRT). The values are the mean of three measurements and error bars represent standard deviation. *P < 0.05 on a student's t-test. (B) Mouse embryonic fibroblasts (MEFs) were obtained from Cux1 knockout embryos (cux1^Z/Z) and wild-type littermates. Levels of CUX1 and DDR gene targets are shown as in A; *in top panel corresponds to non-specific band recognized by CUX1 antibody. (C) Hs578T cells were infected with a retrovirus expressing p110 CUX1 or with the empty vector. RNA was prepared 24 h post-infection and levels of DDR target genes was measured by real-time PCR and normalized to HPRT. The values are the mean of three measurements and error bars represent standard deviation. (D) The promoter regions of target genes were cloned into a luciferase reporter plasmid. Hs578T cells were transfected with each reporter plasmid along with a vector expressing p110 CUX1 or with an empty vector. The values are the mean of three measurements and error bars represent standard deviation.