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. 2012 Feb 8;40(10):4483–4495. doi: 10.1093/nar/gks041

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Effect of CUX1 knockdown on G2/M arrest following IR. (A) Cux1^Z/Z and Cux1 wild-type MEF cells were exposed to 10 Gy IR. Cells were fixed with ethanol 24 h after exposure, stained with Propidium Iodide (PI) and analyzed for cell cycle distribution by flow cytometry. (B) A histogram of the increase in G2 content after IR in MEF cells. *P < 0.05, **P < 0.01, ***P < 0.001; Student's t-test. (C) A histogram of the increase in G2 content after IR in MCF7 cells treated with either CUX1 siRNA or scrambled siRNA.