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. 2012 Feb 8;40(10):4483–4495. doi: 10.1093/nar/gks041

Figure 7.

Figure 7.

Effect of CUX1 knockdown on Rad51 focus formation and DNA break repair. (A) MCF7 cells were transfected with CUX1-specific siRNA before exposure to 5 Gy of IR. Cells were fixed 1 h after exposure and stained by immunofluorescence for Rad51 foci. The proportion of cells displaying five or more foci is shown. Non-irradiated cells are shown as controls. ***P < 0.001; Student's t-test. (B) Cux1Z/Z and wild-type MEFs were irradiated or mock-irradiated with 5 Gy of IR. Cells were fixed 1 h later and stained by immunofluorescence for Rad51 foci. The proportion of cells displaying five or more foci is shown. (C) MCF7 cells were transfected with CUX1-specific siRNA and then exposed to 2 Gy IR, 5 J UV or 10 mm H2O2 for 30 min. At the indicated times, cells were collected and strand breaks quantified by Alkaline Single Cell Gel Electrophoresis (Comet Assay). Comet tail moments were scored for at least 30 cells per conditions. Error bars represent standard error. *P < 0.05, **P < 0.01, ***P < 0.001; Student's t-test. (D) Cux1Z/Z and wild-type MEFs were exposed to IR, UV or H2O2. and DNA breaks were quantified as in (C). In addition, comet tail moments were measured in MEFs maintained at 3 and 20% oxygen with no further treatment.