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. 2012 Feb 10;40(10):e76. doi: 10.1093/nar/gks147

Figure 3.

Figure 3.

Non-selected recombineering in L. reuteri. (a) One hundred colonies derived from transformation with 100 µg oJP577, which upon incorporation yields a rifampicin-resistant phenotype (RpoB H488R), were patched onto MRS agar without antibiotic selection (left—MRS), and onto MRS agar containing rifampicin (right—MRS + RIF). (b) Overview of a selection of genes mutated by non-selected recombineering in the L. reuteri ATCC PTA 6475 chromosome. The locations of the mutated genes on the L. reuteri ATCC PTA 6475 chromosome are indicated based on the closed genome of the closely related strain L. reuteri F275 (NCBI accession number NC_010609). The numbers displayed represent the last four digits of the locus tag (HMPREF0535_XXXX) for each of the genes targeted and the recombineering frequency shown in parentheses. The rpoB gene (DNA directed RNA polymerase; locus tag HMPREF0536_0828) and the ddl gene (d-Ala-d-Ala ligase, locus tag HMPREF0536_1572) are listed using their gene names based on experimental evidence. ND: not determined; ori: origin of replication.