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. 2012 Feb 10;40(10):e76. doi: 10.1093/nar/gks147

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Establishing recombineering in other LAB. (a) The dsDNA sequence of the targeted rpoB region of L. lactis is shown aligned with the encoded amino acids for each codon. On the left are the leading and lagging strand indicated and below oligonucleotides are listed yielding different mismatches, all resulting in a rifampicin-resistant phenotype. The corresponding amino acid changes are listed on the right. (b) Recombineering efficiencies obtained in L. lactis transformed with 100 µg oJP547 or oJP563 and the level of natural rifampicin resistant L. lactis colonies per 10⁹ cells (control). (c) Comparison of recombineering levels between L. reuteri (oJP577), L. lactis (oJP563), L. gasseri (oJP579) and L. plantarum (oJP492). All data shown are averages from three independent experiments and error bars represent standard deviation.