Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 May 15;42(6):597–612. doi: 10.1016/j.ijpara.2012.04.005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Elsevier Ltd.

Open Access under CC BY 3.0 license

PMC Copyright notice

Fig. 8 — Plasmodium berghei subtilisin-like protease 1 (PbSUB1) prefers a P1′ Ser to Asp. Typical progress curves showing cleavage of fluorogenic substrate SERA4st1F-6R (6-iodoacetamido tetramethylrhodamine (6-IATR)-labelled Ac-CITAQDDEEC) by recombinant Plasmodium falciparum SUB1 catalytic domain (rPfSUB1_cat; closed circles) and rPbSUB1_cat (closed triangles), and cleavage of SERA4st1F-6R-S (6-IATR-labelled Ac-CITAQSDEEC) by rPfSUB1_cat (closed squares) and rPbSUB1_cat (closed diamonds). Substitution of the P1′ Asp in SERA4st1F-6R with a Ser in SERA4st1F-6R-S results in a 5.8-fold increase in initial hydrolysis rates by rPbSUB1_cat, but has no significant effect on cleavage by rPfSUB1_cat. Both substrates were used at 0.1 μM final concentration in the digestion reactions.