FIG. 1.

Multiple sequence alignment of maize histone H2B proteins. Multiple protein sequence alignment; histone identification is indicated before each sequence with a suffix to indicate chromosome number (1–10) and gene order on each chromosome (a–c). The top line is the fusion protein H2B-mCherry, and the bottom line is the consensus sequence. Dashes represent gaps introduced by the sequence-alignment software (ClustalW, v1.7). Stop codons (*) are followed by chromosome number, genomic coordinate (Mbp), maize gene ID, and protein length (amino acid number). One gene model (GRMZM2G442555) with histone H2B-like motif was omitted from the alignment because it was predicted to encode a long variant with <50% amino acid sequence identity to the others in the alignment.