FIG. 2.

T-DNA construct, transgene structure, and protein sequence for the fusion protein H2B-mCherry. (A) Diagram of the T-DNA; RB, right border; LB, left border; histone H2B-mCherry transgene, with promoter, Exon 1, and Intron 1 of the maize ubiquitin (Ubi-1) gene; NPTII, Neomycin phosphotransferase gene. (B) Diagram of fusion protein, with maize ubiquitin promoter showing transcription start (+1), locations of primers (TM2_F and TM2_R), and length of the PCR product (695-bp PCR product). (C) Protein sequence of H2B-mCherry, indicating the linker sequence (underlined) separating the H2B and mCherry. PCR, polymerase chain reaction.