FIG. 3.

PCR detection of transgene. PCR evaluating transgene incorporation; individual transformation event lines, corresponding progeny, H2B-mCherry expression, and lane number are indicated along the top of gel. Molecular size markers (λ HindIII) are in lanes 1 and 22, and the position of two marker bands (2023 and 564) is indicated at left. Expression of transgene was verified by microscopy (+or−) for all PCR products. PCRs designed to amplify fragments of H2B-mCherry (lanes 2–19) or control nontransgene sequences—SUN (lane 19) or GAPDH (lane 20)—were carried out with primer sequences described in the Materials and Methods section. Position of the H2B-mCherry 695-bp PCR product is indicated by the arrow.