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H2B-mCherry in postmeiotic early microgametophyte stages leading to mature pollen. Cells (A–L) from fixed anthers were imaged in 3D and displayed as described in Figure 4. Each row shows a single nucleus at the stage indicated on the left. The DAPI image of the nucleus is obscured or difficult to visualize at some of these stages. The position of the nucleus (nucleus) is indicated in the rhodamine channel, and the annulus (J, arrow) is indicated. Scale bars are 10 μm.
