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. Author manuscript; available in PMC: 2013 Jun 29.
Published in final edited form as: Vaccine. 2012 May 15;30(31):4606–4616. doi: 10.1016/j.vaccine.2012.05.005

Figure 1. Influenza rHA proteins produced in various protein expression systems differ in their ability to aggregate into HMWO.

Figure 1

SEC reveals the profile of the oligomeric species contained in the (A) Tni-, (B) HEK-, (C) Sf9-, (D) Nb-, and (E) Sf9 HAT- derived HA preparations from A/Brisbane/59/07. Likewise, the oligomeric species contained in the Tni-, HEK-, Sf9-, Nb-, and Sf9 HAT- derived HA preparations from B/Florida/04/06 are displayed in panels F-J. Molecular weight standards (dotted line) are included as a reference. Fractions containing monomeric HA elute at volumes that correspond to the 66 kDa standard. HMWO fractions (> 669 kDa) contain aggregates that are composed of at least 9 HA monomers.