Figure 5.

Amyloid formation of PGK detected by Thioflavin T fluorescence. Structure formation of acid unfolded PGK was initiated by mixing to pH 2.0 (circles), 3.0 (crosses), or 4.0 (stars) final acidity. (Continuous lines) Fit to the data with the Smoluchowski. The kinetics data were fitted with a coagulation model using size-independent rate constants. The results of the model fit are also indicated. Residuals obtained as the difference between the measured and fitted curves are also shown. Buffer conditions after mixing: 3.6 μM PGK, 9.1 mM Na-citrate, 9.1 mM Na-phosphate, and 182 mM NaCl.