FPLC gel filtration fractionation of solubilized control (solid line) and treated (dashed line) thylakoid membranes. Major fractions are marked as follows: PSII membrane fragments (I), PSII supercomplexes (II), PSI complex (III), PSII core complexes (IV), trimeric LHCII (V), and monomeric minor LHCIIs (VI). Thylakoids were solubilized with 1% α-DM. The top-right inset shows the absorption spectrum of fraction V (solid line) compared with the spectrum of the isoelectric focusing-prepared major LHCII complex (dashed line).